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Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Dudoa (Hydnocarpus alcalae C.DC.) Leaf Extract As a Reducing and Stabilizing Agent

[ Vol. 4 , Issue. 2 ]


Edward K.B. Bragais* and Lynne M. Labaclado*   Pages 112 - 124 ( 13 )


Background and Objective: In this study, dudoa (Hydnocarpus alcalae C.DC.) leaf extract was used as a reducing and stabilizing agent in a novel one-step green synthesis of silver nanoparticles. Dudoa is an endemic plant in the province of Legazpi, Philippines and its seed oil was used as an anti-leprotic drug.

Methods: Therefore, the dudoa leaf extract was used to synthesize silver nanoparticles. Moreover, optimization of various parameters greatly affected the size and morphology of the synthesized AgNPs as indicated by the Ultraviolet-visible (UV-vis) spectrophotometry. The synthesized AgNPs were further characterized using spectral analyses such as XRD, EDX, SEM, FT-IR, TGA and DLS. The antimicrobial activity of synthesized AgNPs was also demonstrated.

Results and Discussion: The synthesized AgNPs exhibited a diffraction pattern and a particle size ranging from 22-48 nm. The AgNPs also showed complete inhibitory and mild reactivity against representative pathogenic gram-positive (S.aureus) and gram-negative (E.coli) bacteria.

Conclusion: Silver nanoparticles were successfully synthesized using H. alcalae leaf extract. Furthermore, this green synthesis approach appeared to be cost-effective, non-toxic, and eco-friendly which is a best alternative to the conventional chemical methods.


Green synthesis, Hydnocarpus alcalae, antimicrobial activity, silver nanoparticles (AgNPs), bioreduction, optimization.


Physical Science Department, College of Science, Pamantasan ng Lungsod ng Maynila, Manila, Physical Science Department, College of Science, Pamantasan ng Lungsod ng Maynila, Manila

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