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Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Dudoa (Hydnocarpus alcalae C.DC.) Leaf Extract As a Reducing and Stabilizing Agent

[ Vol. 4 , Issue. 2 ]

Author(s):

Edward K.B. Bragais* and Lynne M. Labaclado*   Pages 112 - 124 ( 13 )

Abstract:


Background and Objective: In this study, dudoa (Hydnocarpus alcalae C.DC.) leaf extract was used as a reducing and stabilizing agent in a novel one-step green synthesis of silver nanoparticles. Dudoa is an endemic plant in the province of Legazpi, Philippines and its seed oil was used as an anti-leprotic drug.

Methods: Therefore, the dudoa leaf extract was used to synthesize silver nanoparticles. Moreover, optimization of various parameters greatly affected the size and morphology of the synthesized AgNPs as indicated by the Ultraviolet-visible (UV-vis) spectrophotometry. The synthesized AgNPs were further characterized using spectral analyses such as XRD, EDX, SEM, FT-IR, TGA and DLS. The antimicrobial activity of synthesized AgNPs was also demonstrated.

Results and Discussion: The synthesized AgNPs exhibited a diffraction pattern and a particle size ranging from 22-48 nm. The AgNPs also showed complete inhibitory and mild reactivity against representative pathogenic gram-positive (S.aureus) and gram-negative (E.coli) bacteria.

Conclusion: Silver nanoparticles were successfully synthesized using H. alcalae leaf extract. Furthermore, this green synthesis approach appeared to be cost-effective, non-toxic, and eco-friendly which is a best alternative to the conventional chemical methods.

Keywords:

Green synthesis, Hydnocarpus alcalae, antimicrobial activity, silver nanoparticles (AgNPs), bioreduction, optimization.

Affiliation:

Physical Science Department, College of Science, Pamantasan ng Lungsod ng Maynila, Manila, Physical Science Department, College of Science, Pamantasan ng Lungsod ng Maynila, Manila

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